Composite

Part:BBa_K1922012

Designed by: WANG HONGNING   Group: iGEM16_Tongji_Shanghai   (2016-10-14)

hsp70-p53-luciferase

Introduction

Hsp70 promoter works after heat shock, we linked it with luciferase to make it a reporter. Why do we need a reporter? When setting experiment condition in thermotherapy research, it’s important for us to get a feedback when certain temperature has been reached.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 8
    Illegal PstI site found at 359
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 270
    Illegal NheI site found at 2216
    Illegal PstI site found at 8
    Illegal PstI site found at 359
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2537
    Illegal BamHI site found at 415
    Illegal BamHI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 8
    Illegal PstI site found at 359
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 8
    Illegal PstI site found at 359
    Illegal NgoMIV site found at 2571
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 220
    Illegal BsaI.rc site found at 1322
    Illegal SapI site found at 404

Construction

We linked it to vector, after PCR.
"T--Tongji_Shanghai--Parts-h-p-Luci.jpg"

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Categories
Parameters
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