Composite
Part:BBa_K1922012
Designed by: WANG HONGNING Group: iGEM16_Tongji_Shanghai (2016-10-14)
hsp70-p53-luciferase
Introduction
Hsp70 promoter works after heat shock, we linked it with luciferase to make it a reporter. Why do we need a reporter? When setting experiment condition in thermotherapy research, it’s important for us to get a feedback when certain temperature has been reached.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 8
Illegal PstI site found at 359 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 270
Illegal NheI site found at 2216
Illegal PstI site found at 8
Illegal PstI site found at 359 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2537
Illegal BamHI site found at 415
Illegal BamHI site found at 2369 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 8
Illegal PstI site found at 359 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 8
Illegal PstI site found at 359
Illegal NgoMIV site found at 2571 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 220
Illegal BsaI.rc site found at 1322
Illegal SapI site found at 404
Construction
We linked it to vector, after PCR.
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Categories
Parameters
None |